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EC CERTIFICATE

EC Declaration of Conformity Annex (List B)

EC Declaration of Conformity Annex (Others)

CERTIFICATE ISO 13485

InterLabService
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Moscow, Russia, 115035

Phone: +7 (495) 664 2884
Fax: +7 (495) 664 2889

www.interlabservice.ru sales@ilslab.ru
+7 (495) 664 28 84
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AmpliSens
AmpliSens Pyroscreen IHD-screen
Cat.: PMQ-018-50-F-CE
Name: AmpliSens Pyroscreen IHD-screen
Number of tests: 55
Detection Method: Pyrosequencing
Packaging arrangement (PA): Kit with reagents only for amplification step
Content: All reagents are in individual vials (no PCR-tubes)
Shelf life: 9 months

AmpliSens Pyroscreen IHD-screen kit Instruction Manual (version 02.02.2017, update: 27.11.2018)


AmpliSens Pyroscreen IHD-screen kit is an in vitro nucleic acid amplification test based on pyrosequencing technology for genotyping the single-nucleotide polymorphisms (SNPs).

AmpliSens Pyroscreen IHD-screen kit is intended for detection of genetic polymorphisms in genes associated with susceptibility to ischemic heart disease.

Polymorphism for analysis

Locus

Product

Polymorphism

rs

Sequence to Analyze

Analysis

Variants

AMPD1

Adenosine monophosphate deaminase 1

Q12X G>A

rs17602729

AC/TGTGAGTA

reverse

GG

GA

AA

CDKN2A/2B

Cyclin-dependent kinase inhibitors

G>C

rs1333049

TC/GAACTGTTG

reverse

GG

GC

CC

HIF1A

Hypoxia-induced factor 1 alfa

P582S C>T

rs11549465

A/GTGACAACTG

reverse

CC

CT

TT

MMP3

Matrix metalloproteinase 3

5>6

rs3025058

TCCCCCCATCAAAGGA

reverse

5A/5A

5A/6A

6A/6A

APOE

polipoprotein E

C112R T>C

rs429358

GT/CGCGGCCGCC

forward

TT

TC

CC

APOE

polipoprotein E

R158C C>T

rs7412

C/TGCCTGGCAGT

forward

CC

CT

TT

Recommended extraction kits:RIBO-prep(K2-9-Et-100-CE),DNA-sorb-B(K1-2-100-CE). It is possible to use other nucleic acid extraction protocols.

Nesessary reagent kit for sample preparationPEERO-prep(K15-1611-40-CE).

NOTICE: This kit is compatible only with PyroMark Gold Q24 or PyroMark Gold Q96 MD instrument (QIAGEN, Germany).


 
Attention!!! Number of tests includes controls and calibrators (for quantitative tests).

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