Name: AmpliSens® Corynebacterium diphtheriae / tox-genes-FRT PCR kit
Number of tests: 110
Detection Method: FRT
Packaging arrangement (PA): Kit with reagents only for amplification step
Content: All reagents are in individual vials (no PCR-tubes) + Taq-F polymerase (“hot start”)
Shelf life: 12 months
AmpliSens® Corynebacterium diphtheriae / tox-genes-FRT PCR kit is an in vitro nucleic acid amplification test for qualitative detection of Corynebacterium diphtheriae DNA and genes encoding toxins of Corynebacterium diphtheriae and Corynebacterium ulcerans in the biological material (nasopharyngeal swabs, oropharyngeal swabs, swabs from disease sites, germ culture), using real-time hybridization-fluorescence detection of amplified products.
The biological material for study may be taken from patients suspected of diphtheria etiology of the disease (laryngotracheitis, laryngitis, croup); from patients with tonsillitis with a pathological effusion on the tonsils, with suspicion of the pharyngeal (paratonsillar) abscess; infectious mononucleosis; stenosing laryngotracheitis; from persons who were in contact with the source of the infection (for epidemiological reasons); from persons newly admitted to specialized long-stay institutions for children and adults (for prophylactic purposes).
Recommended extraction kit: RIBO-prep (K2-9-Et-100-CE). It is possible to use other nucleic acid extraction protocols.
- Rotor-Gene 3000, Rotor-Gene 6000 (Corbett Research, Australia);
- Rotor-Gene Q (QIAGEN, Germany);
- iCycler iQ5 (Bio-Rad, USA);
- CFX96 (Bio-Rad, USA)
4 or more optical channels are required
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